Cell Acquisition Method Validation by Flow Cytometry for MCTI CIMATEC HDT RNA Vaccine Immunogenicity Evaluation Against SARS-CoV-2

Elen Azevedo da Costa; Carlos Augusto Oliveira Júnior; Alana Costa de Oliveira; Emanuelle de Souza Santos; Milena Botelho Pereira Soares; Bruna Aparecida Souza Machado

doi:10.34178/jbth.v6i3.307

Elen Azevedo da Costa
Carlos Augusto Oliveira Júnior
Alana Costa de Oliveira
Emanuelle de Souza Santos
Milena Botelho Pereira Soares
Bruna Aparecida Souza Machado

DOI: https://doi.org/10.34178/jbth.v6i3.307

Keywords: Flow Cytometry, Validation, COVID-19

Abstract

The COVID-19 pandemic highlighted the importance of developing and improving techniques that can help diagnose and evaluate the disease’s immunological response. Standardizing methods is necessary to ensure the confidence and reproducibility of the results. Therefore, the objective of this work was to carry out a concurrent validation to compare the reference methods for acquiring data used in the evaluation of the immunogenicity of the Vaccine. RNA MCTI CIMATEC HDT, performed by flow cytometry. From the analysis of the cellular profile through the evaluation of cell size and complexity and expression of immunophenotypic markers, no significant differences were found, demonstrating that the methodology used in the BD FacsMelody cytometer at SENAI/CIMATEC is suitable for obtaining the results.