Four Different Cryoprotectors in Preservation of Staphylococcus aureus

Abstract

Preservation associated with the use of cryoprotectants has been one of the most used methods for the long-term storage of microorganisms, mainly because this technique can guarantee the conservation of the original characteristics of the standard strains. The present study aims to evaluate the effectiveness of Skim milk and glycerol as cryoprotectants in preserving the standard strain of Staphylococcus aureus ATCC 6538. Preservation was carried out in freezers at -20°C and -80°C, initially only between December from 2022 to March 2023. Viability and purity tests were performed after one day of freezing and after 3 months to evaluate the method’s ability to maintain the survival and purity of the microorganism. After analyzing the results, the methods were efficient for preservation, confirming that the use of cryoprotective agents in the freezing of Staphylococcus aureus bacteria can be used in laboratory routine.